Figure 7. RT-PCR Confirmation.

a.) The data shows that there is a small increase in σ^H levels of the isogenic mutant, relative to that of the wild type and complemented strain. However, σ^H levels are not sustained in the isogenic mutant, whereas they increase in the wild-type and complemented strain. b.) σ^E levels remain low in all three strains. c.) There is Rv2745c activation in the wild-type and the complemented strain, whereas there is no Rv2745c expression in the isogenic mutant, as expected.