Table 6. Mtb:ΔRv2745c (comp) 60 min post-diamide treatment.
Function | MT # | Symbol | Description | Rv# | M1 | M2 | M3 | Average M Value | Expression Fold Change |
Heat Shock | MT0397 | clpB | ATP-dependent Clp protease, ATP-binding subunit | Rv0384c | 2.454 | 1.961 | 2.321 | 2.245 | 4.741 |
MT3526 | groEL1 | Rv3417c | 1.697 | 1.664 | 1.765 | 1.709 | 3.269 | ||
MT0265 | hsp | heat shock protein, HSP20 family | Rv0251c | 2.973 | 2.698 | 3.026 | 2.899 | 7.459 | |
MT0589 | htpX | heat shock protein HtpX | Rv0563 | 1.759 | 1.651 | 1.804 | 1.738 | 3.336 | |
MT0365 | dnaK | dnaK protein | Rv0350 | 2.158 | 2.336 | 3.394 | 2.629 | 6.187 | |
MT0367 | dnaJ1 | dnaJ protein | Rv0352 | 2.377 | 2.301 | 3.632 | 2.770 | 6.821 | |
Transcription | MT2783 | sigB | RNA polymerase principal sigma factor SigB | Rv2710 | 1.894 | 1.589 | 2.014 | 1.832 | 3.560 |
MT1259 | sigE | RNA polymerase sigma-70 factor, ECF subfamily | Rv1221 | 2.737 | 2.447 | 3.679 | 2.954 | 7.750 | |
MT3320 | sigH | RNA polymerase sigma-70 factor, ECF subfamily | Rv3223c | 3.223 | 3.345 | 4.739 | 3.769 | 13.635 | |
MT1960 | furA | Rv1909c | 2.465 | 2.646 | 2.811 | 2.641 | 6.236 | ||
MT1009 | mprA | Rv0981 | −1.426 | −1.742 | −1.638 | −1.602 | −3.035 | ||
MT1260 | rseA | hypothetical protein | Rv1222 | 2.657 | 2.646 | 3.716 | 3.006 | 8.034 | |
MT3316 | sensor histidine kinase | Rv3220c | 2.100 | 1.708 | 2.563 | 2.124 | 4.358 | ||
Transport | MT2468 | cysA1 | Rv2397c | 3.588 | 3.563 | 3.985 | 3.712 | 13.107 | |
MT2469 | cysW | sulfate ABC transporter, permease protein | Rv2398c | 1.845 | 1.869 | 1.635 | 1.783 | 3.442 | |
MT1519 | Rv1473 | 3.022 | 2.958 | 3.803 | 3.261 | 9.588 | |||
MT2471 | subI | sulfate ABC transporter, sulfate−binding | Rv2400c | 1.098 | 1.467 | 1.011 | 1.192 | 2.285 | |
Detoxification | MT2719 | cadI | Rv2641 | 4.175 | 4.322 | 6.555 | 5.017 | 32.389 | |
MT1517 | trxB1 | thioredoxin | Rv1471 | 3.349 | 3.605 | 5.726 | 4.227 | 18.725 | |
MT4032 | trxB2 | thioredoxin reductase | Rv3913 | 3.218 | 3.290 | 5.195 | 3.901 | 14.936 | |
MT4033 | trxC | thioredoxin | Rv3914 | 2.160 | 2.018 | 3.730 | 2.636 | 6.216 | |
MT1959 | katG | catalase−peroxidase | Rv1908c | 1.713 | 2.331 | 2.757 | 2.267 | 4.814 | |
Lipid metabolism | MT0882 | fadA | Rv0859 | −2.372 | −1.416 | −1.240 | −1.676 | −3.195 | |
Molybdopterin biosynthesis | MT2528 | mobA | molybdopterin−guanine dinucleotide biosynthesis | Rv2453c | 2.375 | 2.000 | 2.914 | 2.430 | 5.388 |
MT3301 | moeB1 | HesA/MoeB/ThiF family protein | Rv3206c | 2.481 | 2.625 | 3.363 | 2.823 | 7.075 | |
Sulfate metabolism | MT1377 | cysM | cysteine synthase | Rv1336 | 2.346 | 2.104 | 2.969 | 2.473 | 5.552 |
MT1324 | cysN | sulfate adenylate transferase, subunit 1 | Rv1286 | 3.611 | 3.698 | 3.389 | 3.566 | 11.841 | |
MT1376.1 | cysO | conserved hypothetical protein | Rv1335 | 2.797 | 2.808 | 4.142 | 3.249 | 9.507 | |
MT0837 | cysA2 | thiosulfate sulfurtransferase | Rv0815c | 1.008 | 1.467 | 1.478 | 1.318 | 2.493 | |
MT3199 | cysA3 | thiosulfate sulfurtransferase | Rv3117 | 1.516 | 1.358 | 1.686 | 1.520 | 2.868 | |
MT2462 | cysH | phosphoadenosine phosphosulfate reductase | Rv2392 | 1.108 | 1.405 | 1.696 | 1.403 | 2.645 | |
Intermediary metabolism | MT3949 | bfrB | Rv3841 | −1.440 | −1.332 | −1.177 | −1.316 | −2.490 | |
MT3969 | ethA | Rv3854c | − | −1.173 | −1.226 | −1.200 | −2.297 | ||
Cell wall associated | MT0870 | lpqS | hypothetical protein | Rv0847 | 1.258 | 1.157 | 1.404 | 1.273 | 2.416 |
MT1379 | murI | glutamate racemase | Rv1338 | 3.322 | 2.855 | 3.729 | 3.302 | 9.863 |
Genes induced and repressed by functional category at 60 minutes post-diamide treatment in the complemented strain. n = 3. MT # and Rv # denote the CDC1551 and the H37Rv gene IDs, respectively.