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. 2014 Apr 4;9(4):e93917. doi: 10.1371/journal.pone.0093917

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© 2014 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) PRRX1 expression was detected by western blot after transfecting pcDNA3.1-PRRX1 into miR-124-overexpressed cells. (B) Cells were followed by treatment as described in Figure 2A. Survival fractions were calculated for each group. The results are presented as the means ±SD of values obtained in 3 independent experiments. ANOVA or Student t tests were used to determine the statistical significance of the differences between groups. Statistical significance(*P<0.05) is indicated vs LV-con and miR-124 group. (C) Representative western blot for the effect of PRRX1 restoration or/and radiation(4Gy) on the expressions of apoptosis related genes(Caspase-3, Bcl-2 andγ-H2AX).