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. 2014 Apr 4;9(4):e93917. doi: 10.1371/journal.pone.0093917

Figure 6. Detecting EMT and stemness-related genes expression by western blot.

Figure 6

Western blot analyzed the EMT-related genes like E-cadherin, ZO-1, Vimentin and N-caherin and stemness-related genes such as ABCG2, SOX2 and Oct4 in miR-124-tranfected cells and miR-124-PRRX1 co-transfected cells compared with control group. Data suggested over-expression of miR-124 could down-regulate Vimentin, N-cadherin, ABCG2, SOX2 and Oct4 expression and up-regulate E-cadherin, ZO-1 expression, while over-expression of PRRX1 could rescue this effect.