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. 2014 Apr 4;9(4):e94127. doi: 10.1371/journal.pone.0094127

Figure 4. Geldanamycin analogues affect glucocorticoid receptors expression.

Figure 4

(A to C) 3T3-L1 preadipocytes were induced to differentiation with or without 17-AAG (100 nM) for 10 days. (A and B) Cell lysates were analyzed by immunoblotting using antibodies against MR, GR and actin asa loading control. (B) The MR and GR expression level was quantified and values were normalized to actin and displayed as mean ± SD (n = 3), *p<0.05, **p<0.01. (C) 3T3-L1 preadipocytes were induced to differentiation and lysed at indicated day with or without 17-AAG treatment (100 nM) for 24 h. (D) Preadipocytes were treated with aldosterone (10 nM) or corticosterone (10 nM) with or without 17-AAG (100 nM) for 3 h before lysis. Cell lysates were then analyzed by immunoblotting using antibodies against MR, GR, and actin as a loading control. (E) White adipose tissue lysates from HFD or HFD+17-DMAG mice were submitted to immunoblotting using antibodies against MR, GR and actin as a loading control. (F) The protein expression levels were quantified and values are given as mean ± SEM (n = 7 HFD, n = 8 HFD+17-DMAG), *p<0.05.