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. 2014 Apr 4;9(4):e94127. doi: 10.1371/journal.pone.0094127

Figure 6. 17-AAG affects adipokines production.

Figure 6

(A) 3T3-L1 preadipocytes were induced or not to differentiation. 24 h supernatants from preadipocytes and mature adipocytes were collected and assessed for adipokines expression using an antibody adipokine array. The cytokine level was determined by chemiluminescence detection and autoradiography. Among the 38 cytokines tested, 8 were up-regulated in mature adipocytes. (B) Each dot of the array was quantified using Image J software. (C) 3T3-L1 preadipocytes were induced to differentiation in presence or absence of 17-AAG (100 nM) from the beginning of the differentiation process. The abundance of different adipokines mRNA was measured by quantitative RT-PCR. (D) 3T3-L1 mature adipocytes were treated or not with 17-AAG (100 nM) for 48 h. The abundance of different adipokines mRNA was measured by quantitative RT-PCR. Given are means (n = 3) relative to GAPDH ± SD, *p<0.05, **p<0.01, ***p<0.001 compared to non-treated mature adipocytes.