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. Author manuscript; available in PMC: 2014 Apr 4.
Published in final edited form as: Biochem J. 2013 Sep 1;454(2):267–274. doi: 10.1042/BJ20130609

Figure 2. FXR increases DGKθ reporter gene activity.

Figure 2

(A) HepG2 cells were transiently transfected with pGL3-DGKθ (pGL3-DGKq), pRL-CMV and pCMX-hFXR, and then treated with 1 nM GW4064 for 16 h. Luciferase activity in lysates isolated from control and GW4064-treated cells was measured by luminometry. The results were normalized to the luciferase activity of Renilla gene and expressed as the fold change in pGL3-DGKθ reporter gene activity over the untreated control group mean and represent the means ± S.E.M. for three separate experiments, each performed in triplicate. *P < 0.05, a statistically significant difference from the untreated control group. (B) HepG2 cells were transiently transfected with wild-type (wt) or mutant (M1 or M2) pGL3-DGKθ, pRL-CMV and pCMX-hFXR expression plasmids. * and # indicate a statistically significant difference (P < 0.05) from untreated control group and untreated FXR group respectively.