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. Author manuscript; available in PMC: 2014 Apr 4.
Published in final edited form as: Biochem J. 2013 Sep 1;454(2):267–274. doi: 10.1042/BJ20130609

Figure 3. GW4064 stimulates FXR recruitment to the DGKθ promoter.

Figure 3

HepG2 cells were incubated with 1 nM GW4064 for 60 min, and then cross-linked with 1 % formaldehyde. The sheared chromatin was harvested and immunoprecipitated with antibodies against FXR or acetyl-histone H3 and recruitment to the DGKθ promoter (− 1000/− 700) assessed by qPCR. DNA purified was quantified by real-time PCR and normalized to the ΔΔCT values of input DNA. The results are expressed as fold change over untreated control. *P < 0.05, a statistically significant difference from untreated control group.