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. 2014 Mar;10(1):25–30.

Table 3.

Antimicrobial activities of J. curcas extracts on coliforms isolated from surface water

Extract Conc. (mg/ml) ZONES OF INHIBITION (mm) ± SD
SHI SAL SER KLE ENT ESC CIT PRO

AQUEOUS 400 13.00 ± 0.01f 15.00 ± 0.00f 16.00 ± 0.00g 14.17 ± 0.15f 9.10 ± 0.10e 14.10 ± 0.01e 15.51 ± 0.01f 13.50 ± 0.10f
200 13.00 ± 0.01f 15.00 ± 0.10f 16.00 ± 0.01g 14.10 ± 0.10e 9.10 ± 0.01e 14.10 ± 0.17e 15.51 ± 0.01f 13.50 ± 0.10f
100 12.01 ± 0.01e 14.00 ± 0.00e 15.10 ± 0.17f 14.01 ± 0.12d 8.01 ± 0.01d 13.02 ± 0.01d 15.50 ± 0.00e 11.50 ± 0.10e
50 12.00 ± 0.01e 14.00 ± 0.06e 15.00 ± 0.00e 10.00 ± 0.01c 8.01 ± 0.01d 11.00 ± 0.06c 12.10 ± 0.10d 10.01 ± 0.01d
25 10.00 ± 0.01d 11.07 ± 0.12d 12.02 ± 0.01c 10.00 ± 0.00c 5.10 ± 0.10b 11.00 ± 0.00c 9.01 ± 0.01c 10.00 ± 0.01d
12.5 7.03 ± 0.01c 8.00 ± 0.01b 11.10 ± 0.01b 5.10 ± 0.01a 5.10 ± 0.10b 8.10 ± 0.10b 9.00 ± 0.01c 6.00 ± 0.01c
Chloroform fraction 12.5 4.60 ± 0.10b 9.00 ± 0.25c 14.50 ± 0.50d 9.00 ± 0.25b 5.80 ± 0.10c 3.60 ± 0.15a 5.10 ± 0.05b 4.50 ± 0.05b
Gentamycin 10 (μg) 0.00a 2.33 ± 1.53a 4.33 ± 1.52 a 18.67 ± 3.06g 4.00 ± 1.00a 24.67 ± 2.51f 3.00 ± 0.25a 0.00a
ACETONe 400 0.0a 0.0a 0.0a 4.10 ± 0.01d 0.0a 0.0a 0.0a 0.0a
200 0.0a 0.0a 0.0a 4.06 ± 0.10d 0.0a 0.0a 0.0a 0.0a
100 0.0a 0.0a 0.0a 2.16 ± 0.04c 0.0a 0.0a 0.0a 0.0a
50 0.0a 0.0a 0.0a 1.02 ± 0.02b 0.0a 0.0a 0.0a 0.0a
25 0.0a 0.0a 0.0a 0.0a 0.0a 0.0a 0.0a 0.0a
12.5 0.0a 0.0a 0.0a 0.0a 0.0a 0.0a 0.0a 0.0a
Chloroform fraction 12.5 4.60 ± 0.10b 9.00 ± 0.25c 14.50 ± 0.50c 9.00 ± 0.25e 5.80 ± 0.10c 3.60 ± 0.15b 5.10 ± 0.05c 4.50 ± 0.05b
Gentamycin 10 (μg) 0.00a 2.33 ± 1.53b 4.33 ± 1.52 b 18.67 ± 3.06f 4.00 ± 1.00b 24.67 ± 2.51c 3.00 ± 0.25b 0.00a
ETHANOL 400 0.0a 3.21 ± 0.02d 0.0a 4.26 ± 0.02d 0.0a 0.0a 0.0a 0.0a
200 0.0a 1.10 ± 0.01b 0.0a 4.18 ± 0.01c 0.0a 0.0a 0.0a 0.0a
100 0.0a 0.0a 0.0a 1.02 ± 0.04b 0.0a 0.0a 0.0a 0.0a
50 0.0a 0.0a 0.0a 0.0a 0.0a 0.0a 0.0a 0.0a
25 0.0a 0.0a 0.0a 0.0a 0.0a 0.0a 0.0a 0.0a
12.5 0.0a 0.0a 0.0a 0.0a 0.0a 0.0a 0.0a 0.0a
Chloroform fraction 12.5 4.60 ± 0.10b 9.00 ± 0.25e 14.50 ± 0.50c 9.00 ± 0.25e 5.80 ± 0.10c 3.60 ± 0.15b 5.10 ± 0.05c 4.50 ± 0.05b
Gentamycin 10 (μg) 0.00a 2.33 ± 1.53c 4.33 ± 1.52b 18.67 ± 3.06f 4.00 ± 1.00b 24.67 ± 2.51c 3.00 ± 0.25b 0.00a

Mean followed by the same letter(s) within the group are not significantly different at p≤0.05. Legend: SHI, Shigella dysenteriae; SAL, Salmonella typhi; SER, Serratia marcescens; KLE, Klebsiella pneumoniae; ENT, Enterobacter aerogenes; ESC, Escherichia coli; CIT, Citrobacter freundii; PRO, Proteus vulgaris; SD, Standard deviation.