Figure 3.

Oscillating-gradient diffusion MRI and histopathological assessment of hippocampal injury at twenty-four hours after neonatal HI (at P8). a) Coronal sections at the level of the hippocampus from a P8 brain show regional decrease in Δ_fADC measurements in the pyramidal cell layer of the ipsilateral injured hippocampus (black arrowheads), compared to the contralateral side. b–c) H&E-stained sections within the areas indicated by the white squares in (a) (sections in (b–c) are rotated by 45° with respect to the Δ_fADC map in (a)) reveal interrupted staining of neuronal cell bodies in the ipsilateral CA1 Py layer, in areas corresponding to the regions of decreased intensity in the Δ_fADC map (black arrowhead). d–e) Fluoro-Jade C labeled sections reveal fluorescent signal located primarily in the Py layer of the ipsilateral hippocampus, with scattered Fluoro-Jade staining in the dentate gyrus at this stage. f–g) High-magnification views of the H&E-stained sections within the area indicated by black squares in (b,c) show neuronal injury and damage in the ipsilateral Py layer, with no evident damage to the contralateral region. h–i) High-magnification views of Fluoro-jade C labeled sections show degenerative changes in the ipsilateral Py layer. Scale bars = 0.5 mm.