Figure 5.

Amyloid fibrils formed by IAPP variants with a free acid C-terminus are much less effective at seeding amyloid formation by hIAPP than amyloid fibrils formed by variants with an amidated C-terminus. ThT fluorescence monitored kinetic experiments: Dots, unseeded hIAPP; Open circles, hIAPP seeded by hIAPP amyloid fibrils; Diamonds, hIAPP seeded by H18Q-hIAPP amyloid fibrils; Triangles, hIAPP seeded by free CT-hIAPP amyloid fibrils; Squares, hIAPP seeded by free H18Q CT-IAPP amyloid fibrils. Seeds were present at 10% concentration in monomer units.