Table 1.
Composition of experimental diets
Ingredient |
Standard bean diet |
Biofortified bean diet |
---|---|---|
g/kg (by formulation) | ||
Biofortified Beans (88 μg Fe/g) |
_ |
400 |
Standard Beans (59 μg Fe/g) |
400 |
_ |
Corn |
350 |
350 |
Corn oil |
30 |
30 |
Dry skim milk |
100 |
100 |
Corn starch |
46.75 |
46.75 |
Vitamin/mineral premix (no Fe) 1 |
70 |
70 |
DL-Methionine |
2.5 |
2.5 |
Choline Chloride |
0.75 |
0.75 |
Total (g) |
1000 |
1000 |
Selected components |
mean ± SEM, n = 5 (by analysis) |
|
Fe (μg Fe/g) 2 |
39.4 ± 0.2b |
52.9 ± 0.9a |
Phytate:Fe molar ratio3 | 8.25 ± 0. 65a | 8.95 ± 0.72a |
1Vitamin and mineral premix provided/kg diet (330002 Chick vitamin mixture; 235001 Salt mix for chick diet; Dyets Inc. Bethlehem, PA).
2Iron concentrations in the diets were determined by an inductively-coupled argon-plasma/atomic emission spectrophotometer (ICAP 61E Thermal Jarrell Ash Trace Analyzer, Jarrell Ash Co. Franklin, MA) following wet ashing.
3Method for determining phytate contents are described in the materials and methods section.
a,bWithin a row, means without a common letter are different, P < 0.05.