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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Mech Dev. 2014 Feb 12;132:28–37. doi: 10.1016/j.mod.2014.02.001

Fig. 2.

Fig. 2

Isolation of X. laevis RFX7 5′ and X. laevis RFX4 5′ clones. (A) Sequences of X. laevis RFX7 5′ (GenBank accession number: KF543241) and X. laevis RFX4 5′ (GenBank accession number: KF543240). The predicted initiation codon is indicated by red characters. (B) RFX7MO and RFX4MO blocked protein translation of RFX7 5′ and RFX4 5′, respectively. MOs and RNAs were co-injected into 2 cell stage embryos and embryos were collected at stage 10. Protein extracts from these embryos were used for immunoblotting.