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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Mech Dev. 2014 Feb 12;132:28–37. doi: 10.1016/j.mod.2014.02.001

Fig. 5.

Fig. 5

The expression of RFX4 is regulated by RFX7 and Foxj1. (A) RFX4 rescued NTD in RFX7 morphants. The bar-graphs show the quantitative assessment of the injections. At least three independent experiments were performed. “n” indicates the number of injected embryos. (B) Five hundred picograms of Foxj1 RNA was injected into a dorsal blastomere of 4-cell stage embryos and the gene expression was examined at stage 14 by whole mount in situ hybridization. β-gal staining (red) indicates the injected side. The number of embryos examined is indicated. (C) Foxj1 RNA was injected into two dorsal blastomeres of 4-cell stage embryos and the gene expression was examined at stage 14 by RT-PCR.