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. Author manuscript; available in PMC: 2014 Apr 5.
Published in final edited form as: FEMS Microbiol Lett. 2012 Jan 6;328(1):78–85. doi: 10.1111/j.1574-6968.2011.02482.x

Figure 3. Expression of ribosomal protein genes in the absence and presence of dcm.

Figure 3

E. coli wild-type bacteria with a plasmid containing an inactive dcm truncation (BW25113/pDcm-9), dcm knockout bacteria containing an inactive dcm truncation (JW1944-2/pDcm-9), and dcm knockout bacteria containing a plasmid with a functional dcm gene (JW1944-2/pDcm-21) were grown to early logarithmic phase and early stationary phase. Total RNA was isolated and converted to cDNA. The levels of rplC and rpsJ were measured by qPCR, and normalized to the levels of malate dehydrogenase (mdh) RNA. The BW25113/pDcm-9 samples were set to a value of one. Error bars represent one standard deviation.