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. 2014 Apr 1;30(4):345–354. doi: 10.1089/aid.2013.0154

FIG. 2.

FIG. 2.

Activation of plasmacytoid dendritic cells (pDCs) and expression of costimulatory molecules. Frequencies of pDCs (CD14− BDCA2+ CD123+; Supplementary Fig. S2A) expressing CD83, CD80, and CD86 (A) and mean fluorescent intensity (MFI) (B) in PBMCs from healthy donors (N=6) cultured overnight in media alone, HIV, 0.1% DMSO, and 0.1% DMSO+HIV. Frequencies of pDCs expressing CD83, CD80, and CD86 (C, E, G, respectively) and MFI (D, F, H, respectively) in PBMCs from the same donors (N=6) cultured overnight in 0.1% DMSO+HIV in the presence or absence of 1 μg/ml HM13N, WM5, WM21, or CQ. In all panels, left plots represent pDC frequencies (C, E, G) or MFI (D, F, H) and right plots represent the relative change caused by each compound compared to 0.1% DMSO+HIV, calculated as [(compound+HIV) – (DMSO+HIV)]/(DMSO+HIV). In all graphs, each symbol represents one individual donor and horizontal and vertical lines indicate medians and IQRs. *p<0.05 using Wilcoxon sign rank test on raw frequencies and MFIs.