Figure 14.

SQR attenuates the stimulatory effect of cysteine on mitochondrial function. Mitochondria were isolated from cultured Hepa1c1c7 cells and subjected to bioenergetic analysis using the Extracellular Flux Analysis method as described (Módis et al., 2013b). (A, B): Mitochondrial function under basal conditions and in the presence of L-cysteine (10 μM) in cultured murine hepatoma cells during the sequential administration of oligomycin (1 μg·mL⁻¹, to inhibit ATP synthase), FCCP (0.3 μM, to induce mitochondrial uncoupling), 2-deoxyglucose (50 mM, to inhibit glycolysis) and antimycin A (2 μg·mL⁻¹, to inhibit Complex III, for determination of non-mitochondrial cellular respiration). (C, D): Mitochondrial function under basal conditions and in the presence of L-cysteine (10 μM) in cultured murine hepatoma cells after SQR silencing (conducted as described in Módis et al., 2013b). Please note that L-cysteine stimulates mitochondrial function in wild-type cells, while it inhibits mitochondrial function in cells with SQR silencing. Data represent mean ± SEM of n = 15 collected from three experiments performed on three different experimental days. Statistical analysis was performed by anova followed by Bonferroni's post hoc test. *P < 0.05 shows significant difference between groups as indicated.