Table 1.
Primers used in the real-time quantitative PCR of genes in chicken samples
| Gene | Accession number | Primer sequences (5′-3′) | Length(bp) |
|---|---|---|---|
|
β-actin |
L08165 |
upstream: CCGAGAGAGAAATTGTGCGTGAC |
166 |
| downstream: TCGGGGCACCTGAACCTCTC | |||
|
FSHβ |
AY029204 |
upstream: GGCTGCGGTGACCATCCTGAATC |
101 |
| downstream: GGCCCCAGTCCTCTCACAGTGCA | |||
|
GnRH-I |
JN609557 |
upstream:TGTCCTCCTGTTCACCGCATCTG, |
222 |
| downstream: TCGATCAGGCTTGCCATGGTTTC | |||
|
LHβ |
HQ872606 |
upstream:GGGGGGAGCGCAGGTGTTG |
220 |
| downstream: CCCGCAGGCCGTGGTGGT | |||
|
AgRP |
AB029443 |
upstream: TCCCCTCGCCGCTGTGTC |
137 |
| downstream: CATGGGAAGGTGGTGCTGATC | |||
|
NPY |
NM_205473 |
upstream: GGAAAGAGATCAAGCCCAGAGAC |
193 |
| downstream: ATGCACTGGGAATGACGCTATG | |||
|
orexin |
AB056748 |
upstream: CGCTGGGCAAGAGGAAGAG |
117 |
| downstream: GGCGCTCCTCACGTTTGC | |||
|
MC4R |
AY545056 |
upstream: GCCAAGAACAAGAACCTCCATT |
150 |
| downstream: TATGGTAAAGCTCTGTGCGTCTG | |||
|
POMC |
NM_001031098 |
upstream: GGCCGAGGCACCCGTGTAC |
141 |
| downstream: GCGGGGTGGTGGGGTGAC | |||
|
LEPR |
NM_204323 |
upstream: TTTGCTGTTGGGCTTTCTTCAC |
148 |
| downstream: AACCAGACCGGCTCCGTACA | |||
|
FSHR |
NM_205079 |
upstream: TCCCACCAATGCCACAGAAC |
155 |
| downstream: TGGGAAGGCTGGAAAACACA | |||
|
LHR |
AB009283 |
upstream: GGGCATGAGCAACGAATCG |
124 |
| downstream: CCGCCTGAGGTTTTTGTTGTC | |||
|
IGF-I |
NM_001004384 |
upstream: TGCTGCTTTTGTGATTTCTTGAA |
138 |
| downstream: AACCAGCTCAGCACCACACAGT | |||
|
fas |
NM_001199487 |
upstream: TTCCCACACACACTGCACATAA |
153 |
| downstream: CACACCGAGAAGAATTGCAGTAA | |||
|
caspase3 |
NM_204725 |
upstream: CCACGCTCAGGGGAAGATGTAT |
173 |
| downstream: CGGTATCTCGGTGGAAGTTCTTA |
|
||
|
bcl2 |
NM_205339 |
upstream: CGCCGCTACCAGAGGGACTTC |
192 |
| downstream: CGCCGCCGAACTCGAAGAAG | |||
|
StAR |
NM_204686 |
upstream: GCCGGACGTGGGTAAGGTGT |
184 |
| downstream: CGCCGTCTCGTGGGTGATC | |||
|
CYP19A1 |
NM_001001761 |
upstream: TGCCAGTTGCCACAGTGCCTATC |
112 |
| downstream: GGCCCAATTCCCATGCAGTATC | |||
|
CYP17A1 |
NM_001001756 |
upstream: CGGGCAGCTTTCAGGCATG |
189 |
| downstream: TGGCCATGATGTTGTGCACGTT | |||
|
PPAR |
AB045597 |
upstream: GCTCCAGGATTGCCAAAGTG |
137 |
| downstream: TCCCCACACACACGACATTCA |