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. 2014 Mar 16;2014:610495. doi: 10.1155/2014/610495

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Copyright © 2014 Leah A. Marquez-Curtis et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

VPA exerts different effects on MSC proliferation and expression of pluripotency markers. (a) Cell proliferation was assayed by measuring the intracellular ATP released by CB-derived MSC after pretreatment with or without (control) 1, 5, or 10 mM VPA. The experiment was performed twice using 6 replicate wells per condition, and *P < 0.05 indicates statistically significant difference relative to control. (b) The expression of the pluripotency genes SOX2 and Oct-4 was evaluated by qRT-PCR using 18S mRNA as internal calibrator. The data is representative of two independent experiments and *P < 0.05 indicates statistically significant difference relative to control.