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. 2014 Mar 17;2014:718769. doi: 10.1155/2014/718769

Figure 2.

Figure 2

α7R agonist increase α7R gene expression in microglia cultures. Primary microglia cultures were stimulated with LPS (10 ng/mL) with or without α7R agonist AR-R 17779 (10 µM) for 4 h. Cells were collected and gene expression of (a) α7, (b) α4, and (c) β2 receptor subunits was investigated using real-time PCR. α7R agonist AR-R 17779 significantly increased α7R expression (a), whereas the α4 receptor subunit was not influenced by the treatment (b). Further, gene expression of β2 receptor subunits was increased by α7R agonist treatment (c). Graph represents pooled data from 4 independent experiments and n = 4/group. Gene expression was normalized to YWHAZ and analyzed using ΔΔCT method. Data are expressed as mean ± SEM, *P < 0.05.