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. 2014 Mar 17;2014:718769. doi: 10.1155/2014/718769

Figure 4.

Figure 4

α7 receptor agonist AR-R 17779 polarize microglia towards Mox phenotype. Primary microglia cultures were stimulated with LPS (10 ng/mL) with or without α7R agonist AR-R 17779 (10 µM) for 4 h. Cells were collected and gene expression of (a) M1 markers CD86 and iNOS, (b) M2 markers CD206 and Arginase 1 (Arg1) and (c) Mox markers heme oxygenase (Hmox1) and sulfiredoxin-1 (Srxn1) was investigated. Treatment with LPS and α7R agonist AR-R 17779 did not influence microglial expression of M1 markers and M2 marker CD206; however, M2 marker Arg1 was downregulated by LPS, and upregulated by the combination of LPS and α7R agonist AR-R 17779 ((a)-(b)). Mox markers Hmox1 and Srxn1 were both upregulated by LPS and α7R agonist AR-R 17779 treatment (c). Graph represents pooled data from 4-5 independent experiments, n = 6–8/group for all except Control AR-R 17779; n = 2. Gene expression was normalized to YWHAZ and analyzed using ΔΔCT method. Data are expressed as mean ± SEM, *P < 0.05, **P < 0.01,***P < 0.001.