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. 2014 Mar 13;2014:481206. doi: 10.1155/2014/481206

Figure 3.

Figure 3

Upregulation of IL-21R in tenocytes in vitro. (a) The levels of mRNA (2−ΔΔCT) for IL-21R were determined by real-time PCR in explanted tenocytes in vitro following stimulation with IL-1, TNF, and in combination. Data are shown as the mean fold change ± SD of triplicate samples compared to no stimulated cells corrected to GAPDH and are representative of experiments using five individual donors of tendon explant tissue. (b) Immunostaining for IL-21R in explant tenocyte cultures stimulated as in (a) confirming changes at protein level.