Figure 7.
Effects of the -3063C>A mutation and consensus HNF-1 binding site on gene expression. Luciferase expression is significantly different between wild-type, -3063C>A, and consensus enhancers when expressed in conjunction with the human wild-type NAGS promoter (A) or the minimal TATA-box promoter (B). All results are an average of three independent experiments that were each carried out in triplicate. Calculated error was from three trials and reported as ±SEM. One asterisk (*) indicates p<0.05 and two asterisks (**) indicate p<0.005.