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. Author manuscript; available in PMC: 2014 Apr 6.
Published in final edited form as: Hum Mutat. 2011 Sep 9;32(10):1153–1160. doi: 10.1002/humu.21553

Figure 8.

Figure 8

Effects of the -3063C>A mutation and consensus HNF-1 binding site on “pull-down” of HNF-1 protein. Amounts of HFN-1 protein pulled down were significantly different between enhancers containing the wild-type, -3063C>A mutated, or consensus HNF-1 binding site (A). Immunoblots (B) show HNF-1 and NF-Y protein following overnight incubation with whole nuclear extract. Values are normalized means from three replicate experiments. Immunoblots are representative of the results obtained by three replicate experiments. Calculated error was from three trials and reported as ±SEM. One asterisk (*) indicates p<0.05 and two asterisks (**) indicate p<0.005.