Table 1.

Primers used to amplify the human NAGS gene for mutation screening

Name	Sequence
Tile1-1f	5- GTG ACT GTG GCT GGT GTG GGT CTC C -3
Tile1-1r	5- GCC AGC TCT TCA TCA CAG CAG GCC ACC -3
Tile 2-1f	5- GAT ACC AGT CCT GCC CTC AGG GAG CC -3
Tile2-1r	5- CAG GGT CAC TCA GCT CAC AAG CCG TGG -3
Tile3-1f	5- GGGTCT GGC CAT GCC ACT GAC GAG C -3
Tile3-1r	5- GTG AGG AGG CGG GTG GGT CAT TTG AGG -3
Tile4-2f	5- AGG TTA AAG AGG GCC GGG CGT GGT GG -3
Tile4-2r	5- GGG TGG TGC TGG CCT TGT CTG TCC TG -3
Tile5-2f	5- GTC TGG GCA ACA TGG TGA ACA CCC GTC -3
Tile5-2r	5- CAG AAC CAC AGC CAT CAG CGC CGT C -3
Tile6-2f	5- CGA CGG CGC TGA TGG CTG TGG TTC TG -3
Tile6-2r	5- CCG TCT CGG TGA CCC TAC CCT TCC AG -3
Tile7-2f	5- CCA TCA CTC CGC GGA CAA GCC CTT CG -3
Tile7-2r	5- CGG GCA GAG TAG CAC GCT CTG AGG AC -3
Tile8-2f	5- TTT GGC GGC GGG TCT GTG CTA CG -3
Tile8-2r	5- TCC TGG ACA GGA GGA GAA GCC GC -3
Tile9-1f	5- CCC ACC ACT CCT CGG CCG TCA TCA CC -3
Tile9-1r	5- CCT TGG TCC GAA GCT GCC CGA CTG CCC -3
Tile10-1f	5- GGG ACG ACT ACC TGG CCT CGC TG -3
Tile10-1r	5- GAG GAC CTA CTG CAT GCT GGG CAC C -3
Tile11-1f	5- CCC ACG CAG CCC ACC TCT CAC AG -3
Tile11-1r	5- CCT GAG TAG AGC CCT GGA AGG CAC CC -3

f-forward and r-reverse