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. Author manuscript; available in PMC: 2014 Apr 6.
Published in final edited form as: Mol Pharm. 2013 Oct 30;10(12):4560–4571. doi: 10.1021/mp400371r

Figure 3.

Figure 3

Representative HPLC chromatograms acquired based on absorbance at 650 nm for: (A) D-Cy5 conjugate, (B) kidney extract, and (C) urine obtained from rabbit 24 h post injection of D-Cy5, (D) Cy5, (E) kidney extract, and (F) urine acquired from rabbit 24 h post injection of the dye. Insets, UV–vis spectra recoded under peaks related to D-Cy5 and Cy5 in samples used as standards, kidney extracts, and urine. Results indicated the presence of D-Cy5, and relatively low levels of free, released dye, in specimens collected from rabbit treated with the conjugate. Relatively low Cy5 levels were detected in animals injected with free Cy5.