Skip to main content
. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Nat Struct Mol Biol. 2014 Mar 16;21(4):397–404. doi: 10.1038/nsmb.2795

Table 2. ITC and stopped-flow analysis of the interaction between aεFab and IgE-Fc or Fcε3-4.

Kdl ITC (μM) Kd2 ITC (μM) Kon(M−1s−1) koff(s−1) kon2(M−1s−1) koff2 (s−1) ΔH1 ITC (Kcal/mol) ΔH2ITC (Kcal/mol)
1aεFab–1IgE-Fc 0.040 (±0.02) – 6.7 (±0.2) × 105 n/m – – −3.5 (±0.1) –
2aεFab–1IgE-Fc 0.076 (±0.07) 1.5 (±1.3) 3.5 (±0.2) × 105 3.2 (± 0.1) 1.2 (±0.2) × 105 n/m −3.5 (±0.1) −1.3 (±0.1)
1aεFab–1Fce3-4 0.050 (±0.02) – 1.0 (±0.1) × 106 n/m – – −3.9 (±0.1) –
2aεFab–1Fce3-4 0.034 (±0.03) 0.98 (±0.45) 1.0 (±0.1) × 106 0.95 (± 0.2) 3.7 (±0.3) × 105 n/m −4.3 (±0.1) −2.3 (±0.1)

n/m not measurable, too slow to measure.