Table 2.

The [3H]-flumazenil binding at various levels of membrane protein in optimum incubation time

Protein concentration (μg)	TB (cpm)	TA (cpm)	(TB×100)/TA
0	0	0	0
50	194.3 ± 12.5	3974	4.85 ± 0.27
100	316 ± 61.7	3974	7.9 ± 1.56
150	457.6 ± 46.7	3974	11.48 ± 1.14
200	517.6 ± 14.04	3974	12.98 ± 0.35
250	551.3 ± 105.02	3974	13.83 ± 2.6
300	580 ± 12.28	3974	14.55 ± 0.3

The values shown are the Mean±SEM of three independent determinations