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. 2014 Winter;13(Suppl):151–160.

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© 2014 by School of Pharmacy, Shaheed Beheshti University of Medical Sciences and Health Services

This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SDS–PAGE analysis of rhINF-β periplasmic expression in E. coli. Lane 1: Chromatine prestained protein ladder (SinaClon, Tehran, Iran), Lane 2: rhINF-β as positive control (ZiferonTM, Zist Daru Danesh, Tehran, Iran), Lane 3: rhINF-β as positive control (Betaseron®, Bayer HealthCare, Germany), Cell lysates were analyzed before IPTG addition (Lane 4) and 1-4 h after addition of 0.2 mM IPTG (Lanes 5-8). The additional band with molecular weight of 18 kDa in periplasmic fraction after induction corresponds to rhINF-β. The arrow indicates position of rhINF-β