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. Author manuscript; available in PMC: 2014 Apr 7.
Published in final edited form as: Environ Microbiol. 2012 Oct 19;14(12):3188–3202. doi: 10.1111/j.1462-2920.2012.02898.x

Figure 2.

Figure 2

In vivo processing of AapA and AapB in E.coli. (A) Signal peptides of AapA/B. The predicted cleavage site is indicated by red letters and the hydrophobic domain is marked by bold letters. (B) Schematic representation of the used plasmids. The plasmids pSVA906 and pSVA914 encode the full length putative prepilin aapA, whereas pSVA907 and pSVA915 encode the full length putative prepilin aapB. pSVA914 and 915 encode additionally to the respective pilin gene also for the type IV prepilin peptidase PibD. (C) Detection of the cleavage of AapA/B by immunoblot analysis using HA-specific antibodies. The fulllength AapA has a size of 17.6 kDa. After pibD expression, AapA displays band at 15 kDa. The fulllength and the processed AapB have a size of 15.9 kDa and 13.9 kDa, respectively.