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Swarming motility assay of aap deletion mutants. The cells were grown to an OD600nm of 1 in Brock medium containing 0.1 % dextrin and 0.1% tryptone and spotted on a Brock plate containing only 0.15% gelrite. The tryptone concentration in the plates was reduced to 0.005% to induce the production of archaella and incubated at 75°C in special metal boxes to prevent evaporation of medium. After seven days, the swarming of the different strains was analysed by scanning the plates.
