Fig. 3.

SB431542 suppresses UVB-induced skin inflammation and T-cell activation in LN and skin. SKH1 mice were irradiated 3× over 1 week with 1 MED UV radiation and treated daily with either acetone vehicle (V) or SB431542 (SB). Quantitation of (A) CD45⁺ and CD3⁺ cells in 7–10 random fields of view (FOV) from at least three stained sections per group. The average of the vehicle-treated control was 29.52 CD45⁺ cells per FOV and 29.62 CD3⁺ cells per FOV. n = 2 for controls and n = 3–4 for UVB IR groups. Error bars = ±SEM. *P < 0.05; **P < 0.01. Scale bar = 100 µm. (B) Percentage of CD4⁺ IFNγ⁺ T cells in viable CD45⁺ leukocytes isolated from SKH1 skin. (C) Cell number of effector CD4⁺ and CD8⁺ T cells (CD44^Hi CD62L^Lo) from SDLN of mice. (D) Cell number of IFNγ⁺ CD4⁺ and CD8⁺ T cells in SDLN of mice treated as in (A). n = 2 for controls and n = 3–4 for UVB-irradiated groups.