Table II. Overview of quantification results for ten proteins of B. subtilis, quantified by AQUA and Hi3 method. The cells were grown either under condition S or under condition CH. Data from the condition CH were not volume-corrected for method comparison.
| Protein | Condition S |
Condition CH |
||||||
|---|---|---|---|---|---|---|---|---|
| AQUA approach |
Hi3 approach |
AQUA approach |
Hi3 approach |
|||||
| Molecules per cell | CV [%] | Molecules per cell | CV [%] | Molecules per cell | CV [%] | Molecules per cell | CV [%] | |
| CitZ | 10,015 | 16.6 | 8373 | 31.7 | 17,751 | 14.6 | 20,578 | 20.4 |
| Icd | 35,106 | 9.4 | 37,100 | 19.9 | 82,649 | 8.2 | 77,080 | 12.6 |
| SdhA | 2773 | 11.8 | 1389 | 25.9 | 8730 | 5.9 | 14,010 | 31.5 |
| Mbl | 499 | 8.1 | 336 | 67.4 | 1854 | 2.6 | 2526 | 36.6 |
| PyrR | 1512 | 4.5 | 688 | 46.5 | 2775 | 2.2 | 2256 | 20.3 |
| Upp | 2,116 | 11.0 | 2,454 | 21.2 | 6285 | 2.3 | 6639 | 23.5 |
| Ald | 1,583 | 1.3 | 844 | 17.5 | 45,804 | 0.9 | 74,422 | 9.8 |
| GapA | 32,406 | 1.1 | 19,205 | 8.8 | 10,649 | 1.2 | 8783 | 28.7 |
| PckA | 123 | 6.4 | 66 | 27.1 | 35,972 | 3.1 | 51,937 | 10.3 |
| RocD | 329 | 4.0 | 336 | 38.9 | 1184 | 5.9 | 113,714 | 6.6 |