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. 2014 Jan 31;13(4):1008–1019. doi: 10.1074/mcp.M113.032631

Table II. Overview of quantification results for ten proteins of B. subtilis, quantified by AQUA and Hi3 method. The cells were grown either under condition S or under condition CH. Data from the condition CH were not volume-corrected for method comparison.

Protein Condition S
Condition CH
AQUA approach
Hi3 approach
AQUA approach
Hi3 approach
Molecules per cell CV [%] Molecules per cell CV [%] Molecules per cell CV [%] Molecules per cell CV [%]
CitZ 10,015 16.6 8373 31.7 17,751 14.6 20,578 20.4
Icd 35,106 9.4 37,100 19.9 82,649 8.2 77,080 12.6
SdhA 2773 11.8 1389 25.9 8730 5.9 14,010 31.5
Mbl 499 8.1 336 67.4 1854 2.6 2526 36.6
PyrR 1512 4.5 688 46.5 2775 2.2 2256 20.3
Upp 2,116 11.0 2,454 21.2 6285 2.3 6639 23.5
Ald 1,583 1.3 844 17.5 45,804 0.9 74,422 9.8
GapA 32,406 1.1 19,205 8.8 10,649 1.2 8783 28.7
PckA 123 6.4 66 27.1 35,972 3.1 51,937 10.3
RocD 329 4.0 336 38.9 1184 5.9 113,714 6.6