Table I. Scaled abundance and coefficient of variation (CV) values for eight nonhuman peptides spiked into human serum for 60-min LC-IMS-TOF MS, 60-min LC-TOF MS, and 100-min LC-LTQ Orbitrap Velos analyses.
| Spiking Level | Peptide | Neutral Mass | Most Abundant Charge State(s) | Peptide Scaled Abundancea and CV Valuesb,c |
||
|---|---|---|---|---|---|---|
| 60-min LC-IMS-TOF MS | 60-min LC-TOF MS | 100-min LC-LTQ Orbitrap Velos | ||||
| 100 pg/ml | Melittin | 2844.75 | 4, 5 | ND | ND | ND |
| 100 pg/ml | Dynorphin A Porcine (Frag 1–13) | 1602.98 | 3 | 1.7 (18) | ND | ND |
| 1 ng/ml | Des Pro Ala Bradykinin | 920.50 | 2 | 21 (12) | ND | ND |
| 1 ng/ml | Leucine Enkephalin | 555.27 | 1 | 23 (10) | ND | ND |
| 10 ng/ml | 3X FLAG Peptide | 2860.14 | 5 | 115 (8) | 125 (20) | ND |
| 10 ng/ml | Substance P | 1346.73 | 2 | 126 (7) | 138 (18) | 112 (19) |
| 100 ng/ml | [Ala92]-Peptide 6 | 2122.18 | 4 | 868 (4) | 848 (11) | 841 (12) |
| 100 ng/ml | Methionine Enkephalin | 573.23 | 1 | 1000 (3) | 1000 (9) | 1000 (10) |
a Peptide abundance values from 3 datasets were averaged and re-scaled to a range of 0 to 1000 for direct instrument comparison (by dividing the most abundant peptide value in each instrument and multiplying by 1000).
b CV values are in parenthesis.
c ND = not detected.