Table II. Limit of detection (LOD) and limit of quantitation (LOQ) values for all of the peptides in 1 μg nonfractionated, 1 μg fractionated, and 4 μg nonfractionated curves.
| Protein | Peptide | 1 μg unfractionated (12 cm/3 μm column) |
1 μg fractionated (12 cm/3 μm column) |
4 μg unfractionated (30 cm/1.9 μm column) |
|||
|---|---|---|---|---|---|---|---|
| LOD (ng/ml) | LOQ (ng/ml) | LOD (ng/ml) | LOQ (ng/ml) | LOD (ng/ml) | LOQ (ng/ml) | ||
| AEBP1 | DTPVLSELPEPVVAR | 78.19 | 234.56 | 4.03 | 12.10 | 15.49 | 46.47 |
| ILNPGEYR | 32.72 | 98.15 | 3.91 | 11.72 | 16.62 | 49.87 | |
| NPFVLGANLNGGER | 122.39 | 367.18 | 5.23 | 15.68 | 9.59 | 28.77 | |
| APR | AGLCQTFVYGGCR | 1.91 | 5.74 | 2.33 | 6.98 | 2.53 | 7.59 |
| BNP | ISSSSGLGCK | 0.68 | 2.05 | 0.24 | 0.72 | 0.63 | 1.89 |
| MVQGSGCFGR | 2.24 | 6.72 | 0.71 | 2.14 | 0.54 | 1.61 | |
| mVQGSGCFGR | 3.51 | 10.54 | 0.54 | 1.61 | 2.13 | 6.40 | |
| MYL3 | AAPAPAPPPEPERPK | 2.79 | 8.37 | 0.58 | 1.74 | 1.12 | 3.37 |
| ALGQNPTQAEVLR | 0.81 | 2.42 | 0.17 | 0.52 | 0.65 | 1.95 | |
| EVEFDASK | 51.65 | 154.96 | 10.88 | 32.63 | 1.09 | 3.26 | |
| HVLATLGER | 1.95 | 5.86 | 0.19 | 0.57 | 0.55 | 1.66 | |
| VVNEECPTITR | 1.62 | 4.87 | 0.39 | 1.16 | 1.03 | 3.10 | |
| HRP | DTIVNELR | 7.03 | 21.10 | 2.70 | 8.10 | 3.52 | 10.56 |
| IL-33 | DFWLHANNK | 33.34 | 100.01 | 4.48 | 13.43 | 12.73 | 38.18 |
| DNHLALIK | 10.67 | 32.02 | 1.41 | 4.23 | 2.97 | 8.92 | |
| TDPGVFIGVK | 12.61 | 37.84 | 0.65 | 1.96 | 5.85 | 17.55 | |
| LEP | INDISHTQSVSAK | 1.39 | 4.16 | 0.81 | 2.43 | 0.46 | 1.37 |
| MBP | YLASASTMDHAR | 6.17 | 18.52 | 0.58 | 1.75 | 0.63 | 1.88 |
| MPO | IANVFTNAFR | 14.28 | 42.85 | 2.46 | 7.37 | 13.09 | 39.26 |
| IPCFLAGDTR | 64.56 | 193.69 | 1.91 | 5.72 | 20.75 | 62.24 | |
| FHL1 | AIVAGDQNVEYK | 4.28 | 12.84 | 0.36 | 1.09 | 1.00 | 3.01 |
| DCFTCSNCK | 8.55 | 25.66 | 1.96 | 5.87 | 1.65 | 4.94 | |
| FCANTCVECR | 2.75 | 8.25 | 0.32 | 0.95 | 1.33 | 3.98 | |
| NPITGFGK | 6.47 | 19.42 | 1.39 | 4.18 | 5.55 | 16.66 | |
| NT-proBNP | EVATEGIR | 2.18 | 6.54 | 0.69 | 2.08 | 0.77 | 2.32 |
| MVLYTLR | 0.81 | 2.44 | 0.26 | 0.79 | 0.36 | 1.08 | |
| VLAIDHLNEDQLR | 1.76 | 5.29 | 0.22 | 0.67 | 0.45 | 1.34 | |
| mVLYTLR | 0.73 | 2.18 | 0.18 | 0.55 | 0.30 | 0.90 | |
| PSA | IVGGWECEK | 3.10 | 9.30 | 1.17 | 3.52 | 0.79 | 2.38 |
| LSEPAELTDAVK | 16.23 | 48.70 | 0.51 | 1.54 | 1.69 | 5.06 | |
| sCD40L | EASSQAPFIASLCLK | 16.40 | 49.21 | 1.21 | 3.62 | 1.41 | 4.23 |
| SLSLLNCEEIK | 12.89 | 38.66 | 0.74 | 2.21 | 1.23 | 3.68 | |
| SQFEGFVK | 7.03 | 21.08 | 0.91 | 2.73 | 6.16 | 18.47 | |
| TTSVLQWAEK | 5.82 | 17.46 | 0.95 | 2.84 | 5.38 | 16.15 | |
| TnI | NIDALSGMEGR | 5.83 | 17.49 | 0.72 | 2.15 | 2.85 | 8.56 |
| NITEIADLTQK | 1.02 | 3.05 | 0.40 | 1.21 | 2.11 | 6.34 | |
| TLLLQIAK | 2.90 | 8.70 | 0.19 | 0.57 | 0.46 | 1.39 | |
| TnT | YEINVLR | 2.10 | 6.31 | 0.99 | 2.96 | 0.75 | 2.25 |
| Tpm | LVIIESDLER | 3.73 | 11.20 | 0.22 | 0.67 | 1.05 | 3.14 |
| QLEDELVSLQK | 16.15 | 48.46 | 1.65 | 4.96 | 15.17 | 45.52 | |
| SIDDLEDELYAQK | 34.06 | 102.18 | 5.65 | 16.96 | 6.56 | 19.67 | |