Table 1.

RAI1 PCR primers and conditions

Exon	Forward primer (5′→3′)	Reverse primer (5′→3′)	Product size, bp	Annealing temp., °C
2	GGGGCTGCTAAGAAGCAAAC	CGCCGAAAACACAAAGCTC	306	56
3.1	GGTGGGTGGGAGGGTGCTTT	TGCTGTCCTGGACGCCGTAG	489	57
3.2	AGTACCACCGAGGCAGCA	TCGGTGCTGTGCAACTCTTA	498	57
3.3	CAGGGTACCCACTTTCCTCA	AGTTCTCCAGGTTTGGCATC	496	57
3.4	CAGGAAACCCTCCATTACCA	GCTGGCTTTTATGCTGCTCT	541	57
3.5	CTCCCTGAGAACCTGCTGTC	CCACGAACTTGGAGAAGCTG	502	57
3.6	AGTCCGTGTCCACCTGTTCT	GTCCGGGAAACAGTCAAAAG	542	57
3.7	TCTCGCTGGAGAACCACAG	CACCTGCCTCCTCCTTCAC	549	57
3.8	CTCAGATGGCATCAGCAAAG	TCAAACCAGCTCTGGACCTT	504	57
3.9	CACTCATCTGCACCAAGGAG	GCCGTGAGAGAACGAGTACA	499	57
3.10	AAGACCTATTGCTCCCTGAA	TTGTCGAGGAGCTTCTTGGT	475	57
3.11	GAGACAGACTCACCCAGCAC	CAGCACCTTGGTCTTCATGG	570	57
3.12	AGTGGCAATGGGGGAGAT	GCCTCGGTTTTGAAACAGTC	530	57
3.13	GGCCTCTCCTGGTAATCCTC	TGTTTCTGTGCCCTTGTCTG	525	57
3.14	GGCCAGTGAGGACAACTCTG	AGGAGAACGAGGACGAGGAT	467	57
3.15	GACGCGTTCACCACCATA	CATCACAGCAGTAGCAGCTC	550	57
3.16	ACACTGCCTCCCCAAAAAG	GTCCTTTGGAACCCCACAC	487	57
4	CCAGCCTGTAAAGCTTGAGG	CTCCAATGCCCTCCTGTTC	197	56
5	TCCTGGCTGCAGACAAAAC	GGGGACTGTGAAGGAGGTG	207	56
6.1	ACTGTGAAGTCCGAGGTCGT	GAAAGTGTCCCCAGGACAAA	420	58
6.2	GGGAGGAAAACCCGTTCC	AGAGGCCAAAGGGTGTCC	549	58
6.3	GGAGCCTTTGGAACAAACC	GTCGCGGTAGCCCCTTAC	489	58
6.4	GGAACATGCTCGCTTCTCC	CAGCTGAATCTGGTCGGAAT	545	62