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. 2014 Mar 19;111(13):4814–4819. doi: 10.1073/pnas.1312801111

Fig. 1.

Fig. 1.

Intercellular localization of GmbHLHm1 in soybean nodule infected cells and yeast. (A and B) Transmission electron microscopy (TEM) analysis of cross-sections of wild-type soybean nodule cells (A) and 26972c yeast expressing GmbHLHm1 (B) after incubation with anti-GmbHLHm1 antiserum (α-bHLHm1, 1:500), followed by 10-nm colloidal gold conjugated with anti-rabbit IgG (1:40). Bact, bacteroid; Nuc, nucleus. (C–F) Confocal images of punctate and nuclear localized GFP::GmbHLHm1 in 26972c cells counterstained (red) with the membrane marker FM4-64. (D) Cells with Hoechst vital DNA staining. (E) N-terminal GFP-tagged GmbHLHm1. (F) Merged images of D and E with DIC image; arrows indicate nuclear localization of GFP signal and Hoechst DNA stain. Nuc, nucleus. (G and H) Identification of full-length and truncated GmbHLHm1 in whole-cell protein fractions of GmbHLHm1-transformed (α-bHLHm1 antiserum) (G) or N-terminal GFP::GmbHLHm1-transformed (using anti-GFP antiserum, α-GFP) 26972c cells (H). In G, the data in lanes 1–3, 4, and 5 represent three individual experiments, respectively. ∆C-term, deletion of the C-terminal TD of GmbHLHm1; Nuc, nuclear enriched fraction; TP, total protein.