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. 2014 Mar 5;111(13):4970–4975. doi: 10.1073/pnas.1323279111

Fig. 2.

Fig. 2.

Requirements for type-I IFN induction and signaling in MERS-CoV clearance. (A) Five days after transduction with 2.5 × 108 pfu of Ad5-hDPP4, mice were intranasally infected with 1 × 105 pfu of MERS-CoV. Weight changes were monitored daily (n = 8 in B6 group; 14 in IFNAR−/− group; and 13 in MyD88−/− group; n= 9 in MAVS−/− group). (B) Virus titers in the lungs were measured at the indicated time points. Titers are expressed as pfu/g tissue (n = 3–4 mice per group per time point). Data are representative of two independent experiments. *, P < 0.05 compared with B6 group; Δ, P values of <0.05 compared with IFNAR−/− group; #, P values of <0.05 compared with MyD88−/− group. (C) Photographs of gross pathological lung specimens isolated from infected mice at day 7 p.i. (D) Sections of paraffin-embedded lungs from Ad5-hDPP4–transduced, infected IFNAR−/− mice were stained with hematoxylin/eosin. (E) Edema (asterisks) and infiltrating eosinophils (arrows) are indicated. (F) Ad5-hDPP4-–transduced mice were treated with 20 μg of poly I:C, 2,000 units of IFN-β, 200 ng of IFN-γ, or PBS in 50 μL of DMEM 6 h before intranasal infection with 1 × 105 pfu of MERS-CoV. Viral titers in lungs were measured at the indicated time points (n = 4 mice per group per time point. Data are representative of three independent experiments. *, P < 0.05 compared with PBS group; Δ, P values of <0.05 compared with poly I:C group; #, P values of <0.05 compared with IFN-β group.