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. 2014 Mar 5;111(13):4970–4975. doi: 10.1073/pnas.1323279111

Fig. 4.

Fig. 4.

Low level of cross-reactivity between MERS-CoV and SARS-CoV. (A–C) Ad5-hDPP4–transduced BALB/c mice were inoculated with 1 × 105 pfu of MERS-CoV or DMEM and rested for 5 wk before infection with 1 × 104 pfu of SARS-CoV in 50 μL of DMEM. Control mice received 300 μL of immune serum from mice previously (5 wk) infected with a sublethal dose of SARS-CoV (500 pfu). Mortality (A) and weight (B) were monitored daily (n = 12 in all groups). (C) To obtain virus titers, lungs were homogenized at the indicated time points and titered on Vero E6 cells. Titers are expressed as pfu/g tissue (n = 4 mice per group per time point). Data are representative of two independent experiments. *, P values of <0.05 compared with DMEM group; Δ, P values of <0.05 compared with MERS-CoV–immunized group. (D–F) BALB/c mice were infected with 500 pfu of SARS-CoV or DMEM and rested for 5 wk before Ad5-hDPP4 transduction and infection with 1 × 105 pfu of MERS-CoV. Control mice received 300 μL of immune serum from mice previously (5 wk) infected with 1 × 105 pfu of MERS-CoV. Mortality (D) and weight (E) were monitored daily (n = 12 in all groups). (F) Virus titers in the lungs were measured at the indicated time points (n = 4 mice per group per time point). Data are representative of two independent experiments. *, P values of <0.05 compared with DMEM group; Δ, P values of <0.05 compared with SARS-CoV–immunized group.