Fig. 3.

Vaccination clone dynamics colored by mutation. (A) Each layer represents a clone. Time is shown by the grid lines on the x axis, and labeled relative to the two vaccination events. The thickness of each layer is proportional to the frequency of that clone at that time point. Each clone is colored based on the average mutation level of the corresponding reads (see color bar for B). Only clones seen in at least two time points are shown here. (B) Histogram of the average mutation level of all of the clones. Each clone is counted once (i.e., clones are not weighted by the number of corresponding reads). Stream graphs are stacked bar charts with moving baselines; if we did not filter out clones in only a single time point, the total thickness of the graph would add up to unity.