Fig. 3.

Validation of newly identified alternatively spliced isoform of Nrxn1α lacking exons 12–18 by RT-PCR. (A) Agarose gel electrophoresis analysis of the PCR product obtained with primers specific to the end of exon 11 and beginning of exon 20 of Nrxn1α. Two major splice variants are identified: a long DNA fragment (∼1,500 bp) corresponding to transcripts containing exons 12–18 as well as a short DNA fragment (∼300 bp) corresponding to transcripts lacking exons 12–18. Note that exon 19 encodes the N terminus of Nrxn1β and is always missing from Nrxn1α mRNAs (12). (B) Sanger sequencing of the short PCR product as obtained in A.