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Ribbon model of holoMb with spin-labeled sites used in DEER measurements (PDB ID code: 2MBW) (43). The spheres indicate the Cα of sites where the nitroxide spin label R1 (Inset) was introduced pairwise to measure the corresponding internitroxide distances (yellow dashed lines). Blue spheres indicate reference sites (see Results). The heme group is shown in stick representation. Arrows next to helix labels indicate the N-to-C terminus direction of each helix.
