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. 2014 Mar 17;111(13):4838–4843. doi: 10.1073/pnas.1321321111

Fig. 3.

Fig. 3.

Engineered bacteria record, remember and report ATC exposure from the mammalian gut. (A) Schematic of experimental design, as described in Methods. (B) Mice were given ATC (0.1 mg/mL) and streptomycin (0.5 mg/mL) in drinking water on day 8. PAS132 cells were administered to the mice via oral gavage on day 9. Streptomycin and ATC were removed from the cage on days 10 and 11, respectively (means ± SD from four mice). (C) Total PAS132 cells in the cI and Cro states. Bars represent the average colony counts from four +ATC mice, and four −ATC mice. (D) The CFU of PAS132 and culturable bacteria (including PAS132) throughout the experiment (means ± SD from eight mice). (E) PAS132 cells were administered on day 9. ATC (0.1 mg/mL) was added to the drinking water on day 10 after streptomycin was removed. ATC was removed from the drinking water on day 11. All PAS132 cells triggered into the cro state within 1 d of ATC exposure, and remembered ATC exposure for more than 7 d (means ± SD from four mice).