Fig. 4.

The E. coli holo-translocon is competent for protein secretion. (A) In vitro translocation assay showing the effect of a light-stimulated PMF on the efficiency of the reaction. SecYEG and HTL proteoliposomes were reconstituted together with BR for the purposes of generating a PMF. The graph shows the percentage of pro-OmpA (pOA) translocated into the liposome interior by SecYEG or the HTL. Values represent the mean of four independent experiments ± SEM. (B) SecY immunoblot of DSP cross-linking of the same proteoliposomes used in A, demonstrating that the organization of liposome-incorporated SecYEG and HTL is maintained.