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. 2014 Mar 18;111(13):5036–5041. doi: 10.1073/pnas.1322360111

Fig. 4.

Fig. 4.

PDE4A is lost from dendritic spines in aged monkey dlPFC. (A and B) In young dlPFC, PDE4A (green arrowheads) is localized next to the SA (asterisk); shown are immunogold and immunoperoxidase, respectively. (Scale bars: 200 nm.) (C) PDE4A is widely expressed in layer III spines in young, but not in aged monkey dlPFC (green circles, PDE4A spines; white circles, unlabeled spines; green rectangle, PDE4A dendrite). (Scale bar: 0.5 μm.) The graphs illustrate percentage of PDE4A spines in 10-y vs. 25-y monkey dlPFC (Upper), averaged from 46-μm2 electron microscopic fields (20 fields from five tissue blocks per animal; SI Materials and Methods) of layer III. Young (Left): PDE4A spines/46 μm2 = 5.95 (± 2.13 SD). Aged (Right): PDE4A spines/46 μm2 = 2.05 (± 1.24 SD).