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. 2014 Mar 18;111(13):4898–4903. doi: 10.1073/pnas.1303559111

Fig. 3.

Fig. 3.

Perturbation of ARF1–GTP activity leads to aberrant hematopoietic phenotypes. (A–H) Whole mounts showing secondary lobe tissue overgrowth marked with DAPI (blue) and increase in mitotically active cells (H3P+, green) in HmldeltaGal4-mediated gartenzwerg (garz) knockdown and Gap69c (arf1GAP) overexpression lymph glands (C, D, G, and H) compared with controls (A, B, E, and F). Graphs show H3P+ cells/lymph gland (O and W). Antp+ (green) niche cells are reduced in garz knockdown and arf1GAP overexpression (N and V) compared with control (M and U) also represented graphically (P and X). Premature differentiation into plasmatocytes (P1, green) (J and R) and crystal cells (ProPO, green) (L and T) in garz knockdown and arf1GAP overexpression compared with controls (I, K, Q, and S), respectively. Red arrows indicate start of the secondary lymph gland lobes. Genotypes are as labeled. C, control; KD, knockdown. [Scale bar, 50 μm (A–H) and 20 μm (I–N and Q–V).]