Table 1.
PCR primers for DNA methylation assays
| Genes | Round # | Primer sequences (5’ to 3’) (forward; reverse) |
|---|---|---|
| Amylase 2a | Touch-down PCR | TTTTATTTTTATTTGGAATGGTG; TCATATTAAACCCAACAAAACC |
| Insulin2 | Touch-down PCR | TTTAAGTGGGATATGGAAAGAGAGATA; ACTACAATTTCCAAACACTTCCCTAATA |
| Glucagon | Nested 1 | TTATATAATGTGGATGAGTGGG; TCTACCCTTCTACACCAAAATAC |
| Glucagon | Nested 2 | TTTGTTTGTTTAGATGAATGATT; TCTACCCTTCTACACCAAAATA |
| Glucagon | Nested 3 | AAGGGATAAGATTTTTAAATGAGA; TCTACCCTTCTACACCAAAATAC |
| Glucagon | Nested 4 | AAGGGATAAGATTTTTAAATGAGA; ACTCTCCAAACTATTTAACCTTACA |
| Somatostatin | Nested 1 | ATTGTTTGGTTTTTGTGGTATG; TCTTCCTTACCTCAAACAACC |
| Somatostatin | Nested 2 | TGGGTGTAGGTTTTTTTTTTTT; TCTTCCTTACCTCAAACAACC |