Table 1.
The results of Ames test on C. paliurus leaf aqueous extract (for the first time). Data are expressed as mean ± SD.
| Dose (μg/plate) | TA97 | TA98 | TA100 | TA102 | ||||
|---|---|---|---|---|---|---|---|---|
| +S9 | −S9 | +S9 | −S9 | +S9 | −S9 | +S9 | −S9 | |
| 5000 | 150.0 ± 13.5 | 147.0 ± 7.5 | 42.7 ± 7.5 | 35.3 ± 4.0 | 179.3 ± 17.1 | 162.3 ± 18.8 | 285.0 ± 15.1 | 261.3 ± 9.9 |
| 1000 | 149.7 ± 26.1 | 151.0 ± 15.1 | 34.3 ± 4.6 | 39.3 ± 7.5 | 169.3 ± 15.6 | 174.7 ± 17.5 | 271.3 ± 10.7 | 275.3 ± 15.5 |
| 200 | 142.3 ± 9.5 | 147.3 ± 25.1 | 32.5 ± 5.5 | 43.0 ± 6.9 | 171.7 ± 11.8 | 175.7 ± 7.0 | 268.3 ± 18.2 | 278.3 ± 12.7 |
| 40 | 141.0 ± 15.5 | 142.7 ± 16.0 | 37.3 ± 8.0 | 39.0 ± 4.6 | 162.7 ± 10.5 | 169.0 ± 17.7 | 277.3 ± 14.2 | 265.7 ± 13.3 |
| 8 | 156.3 ± 12.7 | 145.3 ± 18.6 | 40.7 ± 4.2 | 37.0 ± 4.4 | 172.3 ± 14.3 | 164.3 ± 12.3 | 279.0 ± 13.5 | 267.0 ± 12.3 |
| Spontaneous reverse | 145.7 ± 16.6 | 148.7 ± 14.4 | 41.7 ± 7.6 | 40.0 ± 5.6 | 173.7 ± 13.7 | 170.7 ± 12.2 | 263.7 ± 11.0 | 269.3 ± 23.2 |
| Solvent controls | 154.3 ± 14.8 | 145.7 ± 12.9 | 34.7 ± 5.0 | 37.0 ± 8.5 | 172.3 ± 15.0 | 179.7 ± 10.8 | 273.7 ± 22.5 | 267.3 ± 11.2 |
| Positive controls | 1316.3 ± 130.6 | 1232.0 ± 134.2 | 2664.0 ± 168.7 | 2133.3 ± 107.7 | 2396.7 ± 137.6 | 2426.3 ± 123.6 | 1023.3 ± 127.4 | 2235.3 ± 162.0 |
All the above data were average value of three plates.
Positive controls: TA97 + S9, TA98 + S9, and TA100 + S9 adopted 2-AF (dose: 10.0 μg/plate); TA97 − S9 and TA98 − S9 adopted 9-fluorenone (dose: 0.2 μg/plate); TA100 − S9 adopted NaN3 (dose: 1.5 μg/plate); TA102 + S9 adopted 1,8-mitoxanone (dose: 50.0 μg/plate); TA102 − S9 adopted MMC (dose: 0.5 μg/plate).