Table 3. Binding Free Energy (kJ/mol) for the Thermodynamic Cycle in Scheme 1.

	ΔG1a	ΔG2b	ΔG3c	ΔG4	ΔG4 – ΔG1d	ΔG1 + ΔG2 – 2ΔG3e
puerarin	–12	–30	–32	–10	2	22
daidzin		–38	–28	–22	–10	6
daidzein		–35	–21	–26	–14	–5

^aHH dimerization.

^bBHHP binding.

^c1:1 binding.

^dCooperative effect.

^eTemplating effect.