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Conformational states and subensembles found by the HMM analysis of construct I and construct II. (a) First row: State parameters of the hidden Markov models which are for each state i: the FRET efficiency Ei (abcissa), the state lifetime τi (ordinate), and the equilibrium population πi (disc size). Second and third rows: State decomposition for time scales of 10 and >100 ms. (b) FRET histograms of the subensembles of the states shown in the second row of panel a.
